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anti il 15 inhibitory antibody  (Bio-Techne corporation)


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    Bio-Techne corporation anti il 15 inhibitory antibody
    ( A ) Left panel: uNK cell density in the subluminal stroma was quantified using a standardized immunohistochemistry protocol in LH timed endometrial biopsies (n = 1,997). Right panel: corresponding centile graph. Color code on the left. ( B ) Left panel: example of the tissue distribution of CD56 + uNK cells (brown staining) at LH + 10. Scale bar = 250 μm. Right panel: Pearson’s correlation analysis of stromal cell and uNK cell densities. A total of 80 randomly selected images from 20 biopsies were analyzed. ( C ) Representative images of an eosin stained primary culture decidualized for 8 days incubated for 18 hr with or without uNK cells isolated from luteal phase endometrium. Scale bar = 100 μm. ( D ) SAβG activity in undifferentiated or day eight decidualized EnSCs co-cultured with or without uNK cells in the presence or absence of the apoptosis inhibitor Z-VAD-FMK (Z-VAD, 10 μM) or the granzyme activity inhibitor 3,4-DCI (25 μM). ( E ) Secretion <t>of</t> <t>IL-15</t> secretion was measured every 48 hr in the supernatant of primary EnSCs decidualized for the indicated time-points following pretreatment with vehicle, dasatinib (250 nM) or palbociclib (1 μM). ( F ) SAβG activity in undifferentiated or day eight decidualized EnSCs co-cultured with or without uNK cells in the presence or absence of an IL-15 blocking antibody (1 μg/ml). Data are mean ±SEM of 3 biological replicates unless stated otherwise. Different letters above the error bars indicate that those groups are significantly different from each other at p<0.05. 10.7554/eLife.31274.015 Figure 5—source data 1. uNK cell mediated immune surveillance and clearance of senescent cells.
    Anti Il 15 Inhibitory Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 96/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+il+15+inhibitory+antibody/Human+IL-15+Antibody/pmc05724991-222-26-32
    Average 96 stars, based on 45 article reviews
    anti il 15 inhibitory antibody - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Clearance of senescent decidual cells by uterine natural killer cells in cycling human endometrium"

    Article Title: Clearance of senescent decidual cells by uterine natural killer cells in cycling human endometrium

    Journal: eLife

    doi: 10.7554/eLife.31274

    ( A ) Left panel: uNK cell density in the subluminal stroma was quantified using a standardized immunohistochemistry protocol in LH timed endometrial biopsies (n = 1,997). Right panel: corresponding centile graph. Color code on the left. ( B ) Left panel: example of the tissue distribution of CD56 + uNK cells (brown staining) at LH + 10. Scale bar = 250 μm. Right panel: Pearson’s correlation analysis of stromal cell and uNK cell densities. A total of 80 randomly selected images from 20 biopsies were analyzed. ( C ) Representative images of an eosin stained primary culture decidualized for 8 days incubated for 18 hr with or without uNK cells isolated from luteal phase endometrium. Scale bar = 100 μm. ( D ) SAβG activity in undifferentiated or day eight decidualized EnSCs co-cultured with or without uNK cells in the presence or absence of the apoptosis inhibitor Z-VAD-FMK (Z-VAD, 10 μM) or the granzyme activity inhibitor 3,4-DCI (25 μM). ( E ) Secretion of IL-15 secretion was measured every 48 hr in the supernatant of primary EnSCs decidualized for the indicated time-points following pretreatment with vehicle, dasatinib (250 nM) or palbociclib (1 μM). ( F ) SAβG activity in undifferentiated or day eight decidualized EnSCs co-cultured with or without uNK cells in the presence or absence of an IL-15 blocking antibody (1 μg/ml). Data are mean ±SEM of 3 biological replicates unless stated otherwise. Different letters above the error bars indicate that those groups are significantly different from each other at p<0.05. 10.7554/eLife.31274.015 Figure 5—source data 1. uNK cell mediated immune surveillance and clearance of senescent cells.
    Figure Legend Snippet: ( A ) Left panel: uNK cell density in the subluminal stroma was quantified using a standardized immunohistochemistry protocol in LH timed endometrial biopsies (n = 1,997). Right panel: corresponding centile graph. Color code on the left. ( B ) Left panel: example of the tissue distribution of CD56 + uNK cells (brown staining) at LH + 10. Scale bar = 250 μm. Right panel: Pearson’s correlation analysis of stromal cell and uNK cell densities. A total of 80 randomly selected images from 20 biopsies were analyzed. ( C ) Representative images of an eosin stained primary culture decidualized for 8 days incubated for 18 hr with or without uNK cells isolated from luteal phase endometrium. Scale bar = 100 μm. ( D ) SAβG activity in undifferentiated or day eight decidualized EnSCs co-cultured with or without uNK cells in the presence or absence of the apoptosis inhibitor Z-VAD-FMK (Z-VAD, 10 μM) or the granzyme activity inhibitor 3,4-DCI (25 μM). ( E ) Secretion of IL-15 secretion was measured every 48 hr in the supernatant of primary EnSCs decidualized for the indicated time-points following pretreatment with vehicle, dasatinib (250 nM) or palbociclib (1 μM). ( F ) SAβG activity in undifferentiated or day eight decidualized EnSCs co-cultured with or without uNK cells in the presence or absence of an IL-15 blocking antibody (1 μg/ml). Data are mean ±SEM of 3 biological replicates unless stated otherwise. Different letters above the error bars indicate that those groups are significantly different from each other at p<0.05. 10.7554/eLife.31274.015 Figure 5—source data 1. uNK cell mediated immune surveillance and clearance of senescent cells.

    Techniques Used: Immunohistochemistry, Staining, Incubation, Isolation, Activity Assay, Cell Culture, Blocking Assay

    We propose that rapid endometrial growth during the proliferative phase is important for implantation as it imparts replication stress in a subpopulation of EnSCs. Upon cell cycle exit at G0/G1, this subpopulation of stressed EnSCs do not differentiate into specialist decidual cells but undergo acute cellular senescence and secrete a host of inflammatory mediators (senescence associated secretory phenotype; SASP) involved in endometrial receptivity. In parallel, Il-15 secreted by differentiated decidual cells activates uNK cells, which then target and eliminate senescent cells through granule exocytosis. Systematic clearance of acutely senescent decidual cells by uNK cells not only remodels but also rejuvenates the endometrium at the time of embryo implantation.
    Figure Legend Snippet: We propose that rapid endometrial growth during the proliferative phase is important for implantation as it imparts replication stress in a subpopulation of EnSCs. Upon cell cycle exit at G0/G1, this subpopulation of stressed EnSCs do not differentiate into specialist decidual cells but undergo acute cellular senescence and secrete a host of inflammatory mediators (senescence associated secretory phenotype; SASP) involved in endometrial receptivity. In parallel, Il-15 secreted by differentiated decidual cells activates uNK cells, which then target and eliminate senescent cells through granule exocytosis. Systematic clearance of acutely senescent decidual cells by uNK cells not only remodels but also rejuvenates the endometrium at the time of embryo implantation.

    Techniques Used:

    Related Articles

    Activity Assay:

    Article Title: Clearance of senescent decidual cells by uterine natural killer cells in cycling human endometrium
    Article Snippet: .. Inhibitors were pre-incubated with EnSCs for 1 hr prior to and during co-cultures as follows: Z-VAD-FMK (apoptosis inhibitor) (10 mM), 3,4-DCI (25 mM) (granzyme activity inhibitor), anti-IL-15 inhibitory antibody (1 mg/ml, MAB247, Bio-Techne, Abingdon, UK) and inhibitory anti-NKG2D antibody (10 mg/ml, MAB139, Bio-Techne). siRNA transfection Primary EnSCs were transfected using jetPRIME Polyplus transfection kit (VWR International, Lutterworth, UK) exactly as per manufacturer’s instructions. .. Confluent EnSC monolayers in 24-well plates were downregulated for 18–24 hr in supplemented 2% DMEM/F12 media and transfected with 50 nM siGenome SMARTpool siRNA targeting IL-15 or FOXO1 (Dharmacon, GE Healthcare), or 50 nM MISSION esiRNA targeting CXCL8 (IL-8).

    Article Title: Clearance of senescent decidual cells by uterine natural killer cells in cycling human endometrium
    Article Snippet: .. Inhibitors were pre-incubated with EnSCs for 1 hr prior to and during co-cultures as follows: Z-VAD-FMK (apoptosis inhibitor) (10 μM), 3,4-DCI (25 μM) (granzyme activity inhibitor), anti-IL-15 inhibitory antibody (1 μg/ml, MAB247, Bio-Techne, Abingdon, UK) and inhibitory anti-NKG2D antibody (10 μg/ml, MAB139, Bio-Techne). .. Primary EnSCs were transfected using jetPRIME Polyplus transfection kit (VWR International, Lutterworth, UK) exactly as per manufacturer’s instructions.

    Transfection:

    Article Title: Clearance of senescent decidual cells by uterine natural killer cells in cycling human endometrium
    Article Snippet: .. Inhibitors were pre-incubated with EnSCs for 1 hr prior to and during co-cultures as follows: Z-VAD-FMK (apoptosis inhibitor) (10 mM), 3,4-DCI (25 mM) (granzyme activity inhibitor), anti-IL-15 inhibitory antibody (1 mg/ml, MAB247, Bio-Techne, Abingdon, UK) and inhibitory anti-NKG2D antibody (10 mg/ml, MAB139, Bio-Techne). siRNA transfection Primary EnSCs were transfected using jetPRIME Polyplus transfection kit (VWR International, Lutterworth, UK) exactly as per manufacturer’s instructions. .. Confluent EnSC monolayers in 24-well plates were downregulated for 18–24 hr in supplemented 2% DMEM/F12 media and transfected with 50 nM siGenome SMARTpool siRNA targeting IL-15 or FOXO1 (Dharmacon, GE Healthcare), or 50 nM MISSION esiRNA targeting CXCL8 (IL-8).



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    anti il 15 inhibitory antibody  (Bio-Techne corporation)
    96
    Bio-Techne corporation anti il 15 inhibitory antibody
    ( A ) Left panel: uNK cell density in the subluminal stroma was quantified using a standardized immunohistochemistry protocol in LH timed endometrial biopsies (n = 1,997). Right panel: corresponding centile graph. Color code on the left. ( B ) Left panel: example of the tissue distribution of CD56 + uNK cells (brown staining) at LH + 10. Scale bar = 250 μm. Right panel: Pearson’s correlation analysis of stromal cell and uNK cell densities. A total of 80 randomly selected images from 20 biopsies were analyzed. ( C ) Representative images of an eosin stained primary culture decidualized for 8 days incubated for 18 hr with or without uNK cells isolated from luteal phase endometrium. Scale bar = 100 μm. ( D ) SAβG activity in undifferentiated or day eight decidualized EnSCs co-cultured with or without uNK cells in the presence or absence of the apoptosis inhibitor Z-VAD-FMK (Z-VAD, 10 μM) or the granzyme activity inhibitor 3,4-DCI (25 μM). ( E ) Secretion <t>of</t> <t>IL-15</t> secretion was measured every 48 hr in the supernatant of primary EnSCs decidualized for the indicated time-points following pretreatment with vehicle, dasatinib (250 nM) or palbociclib (1 μM). ( F ) SAβG activity in undifferentiated or day eight decidualized EnSCs co-cultured with or without uNK cells in the presence or absence of an IL-15 blocking antibody (1 μg/ml). Data are mean ±SEM of 3 biological replicates unless stated otherwise. Different letters above the error bars indicate that those groups are significantly different from each other at p<0.05. 10.7554/eLife.31274.015 Figure 5—source data 1. uNK cell mediated immune surveillance and clearance of senescent cells.
    Anti Il 15 Inhibitory Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+il+15+inhibitory+antibody/Human+IL-15+Antibody/pmc05724991-222-26-32
    Average 96 stars, based on 1 article reviews
    anti il 15 inhibitory antibody - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Left panel: uNK cell density in the subluminal stroma was quantified using a standardized immunohistochemistry protocol in LH timed endometrial biopsies (n = 1,997). Right panel: corresponding centile graph. Color code on the left. ( B ) Left panel: example of the tissue distribution of CD56 + uNK cells (brown staining) at LH + 10. Scale bar = 250 μm. Right panel: Pearson’s correlation analysis of stromal cell and uNK cell densities. A total of 80 randomly selected images from 20 biopsies were analyzed. ( C ) Representative images of an eosin stained primary culture decidualized for 8 days incubated for 18 hr with or without uNK cells isolated from luteal phase endometrium. Scale bar = 100 μm. ( D ) SAβG activity in undifferentiated or day eight decidualized EnSCs co-cultured with or without uNK cells in the presence or absence of the apoptosis inhibitor Z-VAD-FMK (Z-VAD, 10 μM) or the granzyme activity inhibitor 3,4-DCI (25 μM). ( E ) Secretion of IL-15 secretion was measured every 48 hr in the supernatant of primary EnSCs decidualized for the indicated time-points following pretreatment with vehicle, dasatinib (250 nM) or palbociclib (1 μM). ( F ) SAβG activity in undifferentiated or day eight decidualized EnSCs co-cultured with or without uNK cells in the presence or absence of an IL-15 blocking antibody (1 μg/ml). Data are mean ±SEM of 3 biological replicates unless stated otherwise. Different letters above the error bars indicate that those groups are significantly different from each other at p<0.05. 10.7554/eLife.31274.015 Figure 5—source data 1. uNK cell mediated immune surveillance and clearance of senescent cells.

    Journal: eLife

    Article Title: Clearance of senescent decidual cells by uterine natural killer cells in cycling human endometrium

    doi: 10.7554/eLife.31274

    Figure Lengend Snippet: ( A ) Left panel: uNK cell density in the subluminal stroma was quantified using a standardized immunohistochemistry protocol in LH timed endometrial biopsies (n = 1,997). Right panel: corresponding centile graph. Color code on the left. ( B ) Left panel: example of the tissue distribution of CD56 + uNK cells (brown staining) at LH + 10. Scale bar = 250 μm. Right panel: Pearson’s correlation analysis of stromal cell and uNK cell densities. A total of 80 randomly selected images from 20 biopsies were analyzed. ( C ) Representative images of an eosin stained primary culture decidualized for 8 days incubated for 18 hr with or without uNK cells isolated from luteal phase endometrium. Scale bar = 100 μm. ( D ) SAβG activity in undifferentiated or day eight decidualized EnSCs co-cultured with or without uNK cells in the presence or absence of the apoptosis inhibitor Z-VAD-FMK (Z-VAD, 10 μM) or the granzyme activity inhibitor 3,4-DCI (25 μM). ( E ) Secretion of IL-15 secretion was measured every 48 hr in the supernatant of primary EnSCs decidualized for the indicated time-points following pretreatment with vehicle, dasatinib (250 nM) or palbociclib (1 μM). ( F ) SAβG activity in undifferentiated or day eight decidualized EnSCs co-cultured with or without uNK cells in the presence or absence of an IL-15 blocking antibody (1 μg/ml). Data are mean ±SEM of 3 biological replicates unless stated otherwise. Different letters above the error bars indicate that those groups are significantly different from each other at p<0.05. 10.7554/eLife.31274.015 Figure 5—source data 1. uNK cell mediated immune surveillance and clearance of senescent cells.

    Article Snippet: Inhibitors were pre-incubated with EnSCs for 1 hr prior to and during co-cultures as follows: Z-VAD-FMK (apoptosis inhibitor) (10 μM), 3,4-DCI (25 μM) (granzyme activity inhibitor), anti-IL-15 inhibitory antibody (1 μg/ml, MAB247, Bio-Techne, Abingdon, UK) and inhibitory anti-NKG2D antibody (10 μg/ml, MAB139, Bio-Techne).

    Techniques: Immunohistochemistry, Staining, Incubation, Isolation, Activity Assay, Cell Culture, Blocking Assay

    We propose that rapid endometrial growth during the proliferative phase is important for implantation as it imparts replication stress in a subpopulation of EnSCs. Upon cell cycle exit at G0/G1, this subpopulation of stressed EnSCs do not differentiate into specialist decidual cells but undergo acute cellular senescence and secrete a host of inflammatory mediators (senescence associated secretory phenotype; SASP) involved in endometrial receptivity. In parallel, Il-15 secreted by differentiated decidual cells activates uNK cells, which then target and eliminate senescent cells through granule exocytosis. Systematic clearance of acutely senescent decidual cells by uNK cells not only remodels but also rejuvenates the endometrium at the time of embryo implantation.

    Journal: eLife

    Article Title: Clearance of senescent decidual cells by uterine natural killer cells in cycling human endometrium

    doi: 10.7554/eLife.31274

    Figure Lengend Snippet: We propose that rapid endometrial growth during the proliferative phase is important for implantation as it imparts replication stress in a subpopulation of EnSCs. Upon cell cycle exit at G0/G1, this subpopulation of stressed EnSCs do not differentiate into specialist decidual cells but undergo acute cellular senescence and secrete a host of inflammatory mediators (senescence associated secretory phenotype; SASP) involved in endometrial receptivity. In parallel, Il-15 secreted by differentiated decidual cells activates uNK cells, which then target and eliminate senescent cells through granule exocytosis. Systematic clearance of acutely senescent decidual cells by uNK cells not only remodels but also rejuvenates the endometrium at the time of embryo implantation.

    Article Snippet: Inhibitors were pre-incubated with EnSCs for 1 hr prior to and during co-cultures as follows: Z-VAD-FMK (apoptosis inhibitor) (10 μM), 3,4-DCI (25 μM) (granzyme activity inhibitor), anti-IL-15 inhibitory antibody (1 μg/ml, MAB247, Bio-Techne, Abingdon, UK) and inhibitory anti-NKG2D antibody (10 μg/ml, MAB139, Bio-Techne).

    Techniques: